Western Blot - Anti-ALDH1A1 Antibody [ARC52440] (A309053)
Western blot analysis of extracts of various cell lines, using Anti-ALDH1A1 Antibody [ARC52440] (A309053) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of A-549 using Anti-ALDH1A1 Antibody [ARC52440] (A309053) at a dilution of 1:800 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HepG2 using Anti-ALDH1A1 Antibody [ARC52440] (A309053) at a dilution of 1:800 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-ALDH1A1 Antibody [ARC52440] (A309053)
Immunoprecipitation analysis of 600µg extracts of mouse testis cells using 3µg of Anti-ALDH1A1 Antibody [ARC52440] (A309053). This Western blot was performed on the immunoprecipitate using Anti-ALDH1A1 Antibody [ARC52440] (A309053) at a dilution of 1:1000.
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