Western blot analysis of Anti-AKT3 Antibody (A8286) sensitivity using recombinant proteins AKT1, AKT2 and AKT3. Lane 1: 0.2µg of human His-AKT1. Lane 2: 0.2µg of human GST-AKT2. Lane 3: 1µg of human GST-AKT3. Lane 4: 0.5µg of human GST-AKT3. Lane 5: 0.2µg of human GST-AKT3.