Anti-AKT1 (phospho Tyr315) Antibody (A16561)

Name: Anti-AKT1 (phospho Tyr315) Antibody
Brand: Antibodies.com
SKU: A16561
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to AKT1 (phospho Tyr315) for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AKT1 (phospho Tyr315) Antibody

Description

Rabbit polyclonal antibody to AKT1 (phospho Tyr315).

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

HepG2.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:100, ICC/IF: 1:100-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide phosphorylated at Y315 of human AKT1.

Sequence

PEYLA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

56 kDa

Predicted MW

48 kDa, 55 kDa, 51 kDa, 54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunohistochemistry - Anti-AKT1 (phospho Tyr315) Antibody (A16561)

AKT1 expression in human brain tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-AKT1 (Phospho Y315) Antibody (A16561) at a dilution of 1:100.

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Immunohistochemistry - Anti-AKT1 (phospho Tyr315) Antibody (A16561)

AKT1 expression in mouse brain tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-AKT1 (Phospho Y315) Antibody (A16561) at a dilution of 1:100.

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Immunohistochemistry - Anti-AKT1 (phospho Tyr315) Antibody (A16561)

AKT1 expression in rat brain tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-AKT1 (Phospho Y315) Antibody (A16561) at a dilution of 1:100.

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Immunofluorescence - Anti-AKT1 (phospho Tyr315) Antibody (A16561)

AKT1 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-AKT1 (Phospho Y315) Antibody (A16561) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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