Anti-AKT1 (phospho Thr308) Antibody [ARC55284] (A307483)

Name: Anti-AKT1 (phospho Thr308) Antibody [ARC55284]
Brand: Antibodies.com
SKU: A307483
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC55284] antibody to AKT1 (phospho Thr308) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AKT1 (phospho Thr308) Antibody [ARC55284]

Description

Rabbit monoclonal [ARC55284] antibody to AKT1 (phospho Thr308).

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T308 of human Phospho-Akt-T308 (NP_005154.2).

Sequence

MKTFC

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55284

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-AKT1 (phospho Thr308) Antibody [ARC55284] (A307483)

Western blot analysis of extracts of Jurkat, using Anti-AKT1 (phospho Thr308) Antibody [ARC55284] (A307483) at 1:2,000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-AKT1 (phospho Thr308) Antibody [ARC55284] (A307483)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-AKT1 (phospho Thr308) Antibody [ARC55284] (A307483) at a dilution of 1:60(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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