Recombinant Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)

Name: Anti-AKT (phospho Ser473) Antibody [RM251]
Brand: Antibodies.com
SKU: A121248
Price: 480.0 USD
Availability: InStock

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$480

Recombinant rabbit monoclonal [RM251] antibody to AKT (phospho Ser473) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

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Recombinant Antibody

Specifications

Name

Anti-AKT (phospho Ser473) Antibody [RM251]

Description

Recombinant rabbit monoclonal [RM251] antibody to AKT (phospho Ser473).

Specificity

This antibody recognizes AKT only when phosphorylated at Ser473.

Applications

WB, IHC

Dilutions

IHC: 1:200-1:500, WB: 1:1,000-1:2,000

Reactivity

Human

Cross Reactivity

This antibody does not cross-react with AKT without phosphorylation at Ser473. This antibody may react with samples from bovine, mouse, and rat, as predicted by immunogen homology.

Immunogen

Synthetic peptide corresponding to human AKT (phospho Ser473).

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM251

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)

Western blot of lysates from 293 cells, either grown in medium with serum, serum starved, or insulin treated, using Anti-AKT (phospho Ser473) Antibody [RM251] at a 1:1,000 dilution or Anti-AKT1 Antibody [RM252] at a 1:1,000 dilution.

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Immunohistochemistry - Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)

Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-AKT (phospho Ser473) Antibody [RM251] at 1:400 dilution.

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