The antibody detects endogenous level of total Akt protein.
Applications
WB, IHC
Reactivity
Human, Mouse
Immunogen
Peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-473) Antibody #21054 (left) or the same antibody preincubated with blocking peptide (right).
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