Anti-AIF Antibody [ARC0015] (A308543)

Name: Anti-AIF Antibody [ARC0015]
Brand: Antibodies.com
SKU: A308543
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0015] antibody to AIF for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Mitochondrial Marker

Specifications

Name

Anti-AIF Antibody [ARC0015]

Description

Rabbit monoclonal [ARC0015] antibody to AIF.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human AIF (O95831).

Sequence

RRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0015

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

67 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIFM1, Apoptosis-inducing factor 1, mitochondrial, PDCD8, Programmed cell death protein 8

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-AIF Antibody [ARC0015] (A308543)

Western blot analysis of extracts of various cell lines, using Anti-AIF Antibody [ARC0015] (A308543) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-AIF Antibody [ARC0015] (A308543)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-AIF Antibody [ARC0015] (A308543) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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