AIF expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AIF Antibody (A82697) at 0.01µg/ml and detected by chemiluminescence.
AIF expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AIF Antibody (A82697) at 0.1µg/ml and detected by chemiluminescence.
AIF expression in Mouse (A), Rat (B) and Pig (C) Heart lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AIF Antibody (A82697) at 0.01µg/ml and detected by chemiluminescence.
AIF expression in Mouse (A) and Rat (B) Kidney lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AIF Antibody (A82697) at 0.03µg/ml and detected by chemiluminescence.
AIF expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-AIF Antibody (A82697) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
AIF expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-AIF Antibody (A82697) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
AIF expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AIF Antibody (A82697) at 5µg/ml and revealed with alkaline phosphatase (AP).
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