Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ADAM 15, Disintegrin and metalloproteinase domain-containing protein 15, MDC-15, MDC15, Metalloprotease RGD disintegrin protein, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, Metargidin
Western Blot - Anti-ADAM15 Antibody [ARC1419] (A308455)
Western blot analysis of extracts of HCT116 cells, using Anti-ADAM15 Antibody [ARC1419] (A308455) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat rectum using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of human colon carcinoma using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse large intestine using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).