Recombinant human monoclonal antibody to TNF alpha.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human TNF alpha Protein (6xHis Tag) (A318337) which can bind Anti-TNF alpha Antibody [TNFa Reference mAb] - Azide free (A318901) in a linear range of 0.3-500 µg/ml.
Flow cytometry analysis with Anti-TNF alpha Antibody [TNFa Reference mAb] - Azide free (A318901) at 1 µg/ml on Expi293 cells transfected with human TNFa (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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