Anti-Actin Antibody [5J11] (A85388)

Immunofluorescent analysis of HeLa cells stained with Anti-Actin Antibody (green) and Anti-Vimentin Antibody (A85421 | red). The Anti-Actin Antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The Anti-Vimentin Antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.

Western blot analysis of tissue and cell lysates probed with Anti-Actin Antibody (red): [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with Anti-UCHL1 Antibody (A85349 | green) - a marker of neuronal lineage cells.

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Anti-Actin Antibody giving a strong clean band at an SDS-PAGE molecular weight of 42 kDa. Lane 16 was probed with Anti-14-3-3 eta Antibody (A85361) which has an SDS-PAGE molecular weight of 28 kDa. Lanes 17 and 18 were probed with two Anti-Vimentin Antibodies (A85424 & A85423, respectively). Note that both Anti-Vimentin Antibodies show strong clean bands at the expected molecular weight of 50 kDa.