Anti-ACTH Antibody [57 (BGN/1388/66)] (A279406)

Anti-ACTH Antibody [57 (BGN/1388/66)]

Mouse monoclonal [57 (BGN/1388/66)] antibody to ACTH.

This antibody recognises 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation.ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output. Mouse anti Human ACTH antibody does not recognize 17-39 ACTH (CLIP). It does cross-reacts with rat N-terminal ACTH.

IHC-P, ELISA

IHC-P 1: 1:100 - 1:200

Human, Rat

Synthetic peptide sequence SYSMEHFRWGKPVGKKRRPVKVYP corresponding to amino acids 1 - 24 of the human ACTH conjugated to Keyhole Limpet Haemocyanin.

SYSMEHFRWGKPVGKKRRPVKVYP

Mouse

Monoclonal

57 (BGN/1388/66)

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human ACTH antibody (Clone 57, BGN/1388/66) recognizes 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation. ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output. Mouse anti Human ACTH antibody does not recognize 17-39 ACTH (CLIP). It does cross-reacts with rat N-terminal ACTH.

Corticotropin-lipotropin, Pro-opiomelanocortin