Anti-Acetylcholinesterase Antibody (A83194)

Acetylcholinesterase expression in Jurkat (A) and HepG2 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Acetylcholinesterase Antibody (A83194) at 0.3µg/ml (A) or 0.5µg/ml (B) and detected by chemiluminescence.

Acetylcholinesterase expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Acetylcholinesterase Antibody (A83194) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Acetylcholinesterase expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Acetylcholinesterase Antibody (A83194) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.