Anti-A RAF Antibody [ARC1782] (A308522)

Name: Anti-A RAF Antibody [ARC1782]
Brand: Antibodies.com
SKU: A308522
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1782] antibody to A RAF for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-A RAF Antibody [ARC1782]

Description

Rabbit monoclonal [ARC1782] antibody to A RAF.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 166-290 of human A-RAF (P10398).

Sequence

RQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1782

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ARAF, ARAF1, PKS, PKS2, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, Serine/threonine-protein kinase A-Raf

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-A RAF Antibody [ARC1782] (A308522)

Western blot analysis of extracts of various cell lines, using Anti-A RAF Antibody [ARC1782] (A308522) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-A RAF Antibody [ARC1782] (A308522)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-A RAF Antibody [ARC1782] (A308522) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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