Recombinant Anti-5-hydroxymethylcytosine Antibody [RM236] (A121325)

ELISA of single stranded DNA using Anti-5-hydroxymethylcytosine Antibody [RM236]. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of Anti-5-hydroxymethylcytosine Antibody [RM236] was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Dot blot of double stranded DNA using Anti-5-hydroxymethylcytosine Antibody [RM236]. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and un-methylated DNA. The pre-spotted membrane was then blotted with Anti-5-hydroxymethylcytosine Antibody [RM236].

Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections, using Anti-5-hydroxymethylcytosine Antibody [RM236].

Immunocytochemical staining of HeLa cells using 0.5µg/ml Anti-5-hydroxymethylcytosine Antibody [RM236] (red). Actin filaments have been labeled with fluorescein phalloidin (green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (-20°C) before treatment with 2 N HCl for 30 min at 37°C to denature DNA.