Recombinant Anti-5-hydroxymethylcytosine Antibody [RM231] (A121326)

ELISA of single stranded DNA using Anti-5-hydroxymethylcytosine Antibody [RM231]. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of Anti-5-hydroxymethylcytosine Antibody [RM231] was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Dot blot of double stranded DNA using Anti-5-hydroxymethylcytosine Antibody [RM231]. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and un-methylated DNA. The pre-spotted membrane was then blotted with Anti-5-hydroxymethylcytosine Antibody [RM231].

Immunocytochemical staining of HeLa cells using Anti-5-hydroxymethylcytosine Antibody [RM231] (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).

Direct ELISA of HeLa cell genomic DNA using Anti-5-hydroxymethylcytosine Antibody [RM231]. The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. 1µg/ml or 3µg/ml of Anti-5-hydroxymethylcytosine Antibody [RM231] was used as the primary antibody, and a HRP conjugated anti-rabbit IgG as the secondary antibody.