Anti-160 kD Neurofilament Medium Antibody (A88233)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-160 kD Neurofilament Medium Antibody (A88233) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-160 kD Neurofilament Medium Antibody (A88233) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-160 kD Neurofilament Medium Antibody (A88233) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of SH-SY5Y cells using Anti-160 kD Neurofilament Medium Antibody (A88233) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).