Universal IL-10 ELISA Kit (A117618)

Universal IL-10 ELISA Kit

Universal IL-10 ELISA Kit is a Competive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal IL-10 in serum, plasma and cell culture supernates.

Competitive (quantitative)

Universal IL-10 ELISA Kit (A117618) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal IL-10 in serum, plasma and cell culture supernates. The 96-well microtiter plate has been pre-coated with IL-10 antigen. During the incubation, IL-10 present in the samples or standards competes with the fixed amount of immobilized IL-10 for binding sites on the Biotinylated Anti-IL-10 Antibody. The more IL-10 present in a sample or standard, the less Biotinylated Anti-IL-10 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-IL-10 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of IL-10 present in each sample or standard. The concentration of IL-10 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Serum, plasma and cell culture supernates.

0.93 ng/ml

2.47-200 ng/ml

2.5h

100µl

Universal

Intra-assay CV: <4.7%, Inter-assay CV: <6.2%.

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

This product is for research use only. It is not intended for diagnostic or therapeutic use.