SKP1A Cell Based ELISA Kit (A103115)

SKP1A Cell Based ELISA Kit

Cell-based (qualitative)

This SKP1A Cell Based ELISA Kit allows for the detection of SKP1A and the effects that certain stimulation conditions have on SKP1A expression in different cell lines. Qualitative determination of SKP1A concentration is achieved by an indirect ELISA format. In essence, the SKP1A is captured by Anti-SKP1A Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SKP1A Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Cyclin-A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SIII, TCEB1L