Rat IFN gamma ELISA Kit (A117515)

Rat IFN gamma ELISA Kit

Rat IFN gamma ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat IFN gamma in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Rat IFN gamma ELISA Kit (A117515) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat IFN gamma in serum, plasma and cell culture supernates. An antibody specific for IFN gamma has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IFN gamma present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IFN gamma Antibody, which binds the captured IFN gamma present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IFN gamma captured in each well. The concentration of IFN gamma can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

18.1 pg/ml

78-5000 pg/ml

4 hours

100µl

Rat

Intra-assay CV: <3.1%, Inter-assay CV: <7.1%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-IFN gamma Antibody (A329462) as the capture antibody, Anti-IFN gamma Antibody (A329463) as the detection antibody, and Recombinant Rat IFN gamma Protein (C-terminal Human Fc and His Tag) (A331640) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

IFN-gamma, IFNG, Immune interferon, Interferon gamma