Rat CXCL1 ELISA Kit (A1610)

Rat CXCL1 ELISA Kit

Sandwich (quantitative)

Rat CXCL1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat CXCL1 in the samples. A monoclonal antibody specific for rat CXCL1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CXCL1, and CXCL1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

15.63 - 1000 pg/ml

This kit can assay both natural and recombinant rat CXCL1.

Rat

This assay has shown no significant cross-reactivity.

100% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

10 kDa interferon gamma-induced protein, C-X-C motif chemokine 1, C-X-C motif chemokine 10, CXCL10, Gamma-IP10, GRO, GRO-alpha(1-73, GRO1, GROA, Growth-regulated alpha protein, INP10, IP-10, Melanoma growth stimulatory activity, MGSA, NAP-3, Neutrophil-activating protein 3, SCYB1, SCYB10, Small-inducible cytokine B10