Rabbit Anti-Polyethylene Glycol IgM ELISA Kit (A326392)

Rabbit Anti-Polyethylene Glycol IgM ELISA Kit

Rabbit Anti-Polyethylene Glycol IgM ELISA Kit is a indirect Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rabbit Anti-Polyethylene Glycol IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Indirect (quantitative)

Rabbit Anti-Polyethylene Glycol IgM ELISA Kit (A326392) employs the Indirect ELISA technique for the quantitative measurement of rabbit Anti-Polyethylene Glycol IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.. The 96-well microtiter plate has been pre-coated with Anti-Polyethylene Glycol IgM antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of Anti-Polyethylene Glycol IgM sample captured on the plate. The concentration of Anti-Polyethylene Glycol IgM can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

0.094 ng/ml

3 hours

Rabbit

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

This product is for research use only. It is not intended for diagnostic or therapeutic use.