Rabbit CD4 ELISA Kit (A327120)

$764

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Rabbit CD4 ELISA Kit
Description
Rabbit CD4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rabbit CD4 in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Rabbit CD4 ELISA Kit (A327120) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rabbit CD4 in serum, plasma, and other biological fluids. An antibody specific for CD4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CD4 Antibody, which binds the captured CD4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CD4 captured in each well. The concentration of CD4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, and other biological fluids.
Sensitivity
0.94 ng/ml
Product Range
1.56-100 ng/ml
Assay Time
4h 30m
Reactivity
Rabbit
Cross Reactivity
No significant cross-reactivity or interference between rabbit CD4 and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 91% - 105% 96%
EDTA Plasma 8 94% - 111% 101%
Cell Culture Media 8 85% - 100% 91%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 86-99% 87-101% 88-100% 83-94%
EDTA Plasma (n=5) 96-110% 95-112% 92-107% 91-107%
Cell culture media (n=5) 89-102% 86-97% 87-99% 84-96%
Precision
Intra-assay CV: 4.8%, Inter-assay CV: 4.76%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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