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PTEN Cell Based ELISA Kit (A103257)

$570

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.
Name
PTEN Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This PTEN Cell Based ELISA Kit allows for the detection of PTEN and the effects that certain stimulation conditions have on PTEN expression in different cell lines. Qualitative determination of PTEN concentration is achieved by an indirect ELISA format. In essence, the PTEN is captured by Anti-PTEN Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PTEN Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Inositol polyphosphate 3-phosphatase, MMAC1, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, TEP1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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