Porcine FGF2 ELISA Kit (A327093)

Porcine FGF2 ELISA Kit

Porcine FGF2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of porcine FGF2 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Porcine FGF2 ELISA Kit (A327093) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine FGF2 in serum, plasma, and other biological fluids. An antibody specific for FGF2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the FGF2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-FGF2 Antibody, which binds the captured FGF2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of FGF2 captured in each well. The concentration of FGF2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

9.38 pg/ml

15.63-1000 pg/ml

Porcine

No significant cross-reactivity or interference between porcine FGF2 and analogues was observed.

Intra-assay CV: 5.1%, Inter-assay CV: 5.24%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Basic fibroblast growth factor, bFGF, FGF-2, FGF-20, FGF20, FGFB, Fibroblast growth factor 2, Fibroblast growth factor 20, HBGF-2, Heparin-binding growth factor 2