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Mouse TrkB ELISA Kit (A726)

$795

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Mouse TrkB ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This TrkB enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TrkB Antibody. Standards or samples are then added to the microtire plate wells and TrkB, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TrkB present in the sample, Anti-TrkB Antibody (Biotin) is added to each well to "sandwich" the TrkB immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TrkB will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Tissue lysates and cell culture supernatants.
Sensitivity
< 5 pg/ml
Product Range
93.8-6,000 pg/ml
Reactivity
Mouse
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, NTRK2, Trk-B, TrkB tyrosine kinase, Tropomyosin-related kinase B
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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