Mouse Osteopontin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse OPN in the samples. A monoclonal antibody specific for mouse OPN has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for OPN, and OPN present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPN bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.05 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both natural and recombinant mouse OPN.
Reactivity
Mouse
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
Storage
Store at 2 - 8°C.
Synonyms
BNSP, Bone sialoprotein 1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1, Urinary stone protein, Uropontin
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
