Mouse IL-6 ELISA Kit (Small Sample Volume) (A323188)

Name: Mouse IL-6 ELISA Kit (Small Sample Volume)
Brand: Antibodies.com
SKU: A323188
Price: 495.0 USD
Availability: InStock

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Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Mouse IL-6 ELISA Kit (Small Sample Volume)

Description

Mouse G-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse G-CSF in serum, plasma, and other biological fluids. This ELISA kit only requires 25µl of sample volume(s).

Assay Type

Sandwich (quantitative)

Principle of Assay

Mouse IL-6 ELISA Kit (A323188) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse IL-6 in serum, plasma, and other biological fluids. An antibody specific for IL-6 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-6 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-6 Antibody, which binds the captured IL-6 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-6 captured in each well. The concentration of IL-6 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colormetric

Instrument

Colorimetric Microplate Reader

Sample Type

Serum, plasma, and other biological fluids.

Sensitivity

18.75 pg/ml

Product Range

31.25-2000 pg/ml

Assay Time

3h 30m

Reactivity

Mouse

Cross Reactivity

No significant cross-reactivity or interference between mouse IL-6 and analogues was observed.

Recovery

Sample Type	n	Range	Average
Serum	8	92% - 101%	98%
EDTA Plasma	8	90% - 103%	96%
Cell Culture Media	8	90% - 106%	98%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	90% - 101%	93% - 102%	84% - 97%	93% - 100%
EDTA Plasma (n=5)	89% - 100%	94% - 106%	100% - 110%	86% - 100%
Cell Culture Media (n=5)	86% - 101%	85% - 97%	89% - 101%	85% - 99%

Precision

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Storage

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Synonyms

B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL6, Interferon beta-2, Interleukin-6

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.