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Mouse Granzyme B ELISA Kit (A1563)

$530

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 10-14 business days.
Name
Mouse Granzyme B ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse Granzyme B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Granzyme B in the samples. A monoclonal antibody specific for mouse Granzyme B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Granzyme B, and Granzyme B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Granzyme B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
9.91 pg/ml
Product Range
312.50 - 20000 pg/ml
Specificity
This kit can assay both natural and recombinant mouse Granzyme B.
Reactivity
Mouse
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
99% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
C11, Cathepsin G-like 1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, GZMB, Lymphocyte protease
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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