Mouse CCL2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CCL2 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse CCL2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL2 in the samples. A monoclonal antibody specific for mouse CCL2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL2, and CCL2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
20.54 pg/ml
Product Range
62.50 - 4000 pg/ml
Specificity
This kit can assay both native and recombinant mouse CCL2.
Reactivity
Mouse
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
100% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
C-C motif chemokine 2, HC11, MCAF, MCP-1, MCP1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
