Monkey Uteroglobin ELISA Kit (A327051)

Monkey Uteroglobin ELISA Kit

Monkey Uteroglobin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey Uteroglobin in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Monkey Uteroglobin ELISA Kit (A327051) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Uteroglobin in serum, plasma, and other biological fluids. An antibody specific for Uteroglobin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Uteroglobin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Uteroglobin Antibody, which binds the captured Uteroglobin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Uteroglobin captured in each well. The concentration of Uteroglobin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

37.50 pg/ml

62.50-4000 pg/ml

Monkey

No significant cross-reactivity or interference between monkey Uteroglobin and analogues was observed.

Intra-assay CV: 4.9%, Inter-assay CV: 5.33%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

CC10, CCPBP, CCSP, Club cell phospholipid-binding protein, Club cells 10 kDa secretory protein, SCGB1A1, Secretoglobin family 1A member 1, UGB, UP-1, UP1, Urinary protein 1, Urine protein 1

Information online is representative. Always refer to the Product Manual inside the kit.