Monkey Granzyme A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey Granzyme A in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Monkey Granzyme A ELISA Kit (A327082) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Granzyme A in serum, plasma, and other biological fluids. An antibody specific for Granzyme A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Granzyme A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Granzyme A Antibody, which binds the captured Granzyme A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Granzyme A captured in each well. The concentration of Granzyme A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, and other biological fluids.
Sensitivity
9.38 pg/ml
Product Range
15.63-1000 pg/ml
Assay Time
3h 30m
Reactivity
Monkey
Cross Reactivity
No significant cross-reactivity or interference between monkey Granzyme A and analogues was observed.
Recovery
Linearity
Sample Type
1:2
1:4
1:8
1:16
Serum (n=5)
92-110%
91-104%
89-101%
96-113%
EDTA Plasma (n=5)
87-100%
84-100%
83-95%
83-98%
Cell culture media (n=5)
91-106%
88-102%
87-102%
89-100%
Precision
Intra-assay CV: 5.33%, Inter-assay CV: 5.13%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
