Principle of Assay
Monkey beta Crosslaps/beta CTx ELISA Kit (A327083) employs the competitive enzyme immunoassay technique for the quantitative measurement of monkey beta Crosslaps/beta CTx in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with beta Crosslaps/beta CTx antigen. During the incubation, beta Crosslaps/beta CTx present in the samples or standards competes with the fixed amount of immobilized beta Crosslaps/beta CTx for binding sites on the Biotinylated Anti-beta Crosslaps/beta CTx Antibody. The more beta Crosslaps/beta CTx present in a sample or standard, the less Biotinylated Anti-beta Crosslaps/beta CTx Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-beta Crosslaps/beta CTx Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of beta Crosslaps/beta CTx present in each sample or standard. The concentration of beta Crosslaps/beta CTx can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
