Human YKL-40 / CHI3L1 ELISA Kit (A311360)

Human YKL-40 / CHI3L1 ELISA Kit

Human YKL-40 / CHI3L1 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human YKL-40 / CHI3L1 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Human YKL-40 / CHI3L1 ELISA Kit (A311360) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human YKL-40 / CHI3L1 in An antibody specific for YKL-40 / CHI3L1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the YKL-40 / CHI3L1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-YKL-40 / CHI3L1 Antibody, which also binds the YKL-40 / CHI3L1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-YKL-40 / CHI3L1 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-YKL-40 / CHI3L1 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of YKL-40 / CHI3L1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of YKL-40 / CHI3L1 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Microplate Reader

Serum, plasma or other biological fluids.

0.61 ng/ml

15-240 ng/ml

1h 30m

Human

Intra-assay CV: 5.2%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!