Principle of Assay
Human vWF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human vWF in the samples. A monoclonal antibody specific for human vWF has been immobilized onto microwells. Standard or samples are pipetted into the wells, and vWF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for vWF is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of vWF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
