Human VEGF Receptor 1 ELISA Kit (A1536)

Human VEGF Receptor 1 ELISA Kit

Sandwich (quantitative)

Human VEGF R1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VEGF R1 in the samples. A monoclonal antibody specific for human VEGF R1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for VEGF R1, and VEGF R1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF R1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

156.25 - 10000 pg/ml

This kit can assay both natural and recombinant human VEGF R1.

Human

This assay has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

FLT, FLT-1, FLT1, Fms-like tyrosine kinase 1, FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGFR-1, VEGFR1