Human TNF alpha Matched Antibody Pair Kit includes an unlabeled monoclonal capture antibody, biotin-labeled monoclonal detection antibody, Streptavidin-HRP, recombinant human TNF alpha ELISA standard, and standard reconstitution buffer. This matched antibody pair kit can be used to quantify native and recombinant human TNF alpha. Matched antibody pair kits are ideal for economical ELISA and ELISA-based assay development.
Assay Type
Sandwich (quantitative)
Detection Type
Colorimetric
Sample Type
Serum, plasma, and cell culture supernatant.
Product Range
4-400 pg/ml
Specificity
This matched antibody pair kit utilizes a pair of antibodies to specifically recognize human TNF alpha.
Reactivity
Human
Recovery
General Notes
It may be necessary to optimize the reagents in this matched antibody pair kit for your sample type, immunoassay format, or instrumentation. The protocol manual outlines the recommended assay procedure to run a standard 96-well microplate sandwich ELISA using HRP/TMB system for colorimetric quantitative detection of human TNF alpha.
Storage
Shipped at +4°C. Store the ELISA standard at -20°C. Store all other reagents at +4°C.