Human Serpin E1 ELISA Kit (A1512)

Human Serpin E1 ELISA Kit

Sandwich (quantitative)

Human Serpin E1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Serpin E1 in the samples. A monoclonal antibody specific for human Serpin E1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Serpin E1, and Serpin E1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Serpin E1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

312.50 - 20000 pg/ml

This kit can assay both natural and recombinant human Serpin E1.

Human

This assay has shown no significant cross-reactivity.

90% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

Endothelial plasminogen activator inhibitor, PAI, PAI-1, PAI1, PLANH1, Plasminogen activator inhibitor 1, SERPINE1