Human SCF ELISA Kit (A1510)

Human SCF ELISA Kit

Sandwich (quantitative)

Human SCF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human SCF in the samples. A monoclonal antibody specific for human SCF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for SCF, and SCF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of SCF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human SCF.

Human

This assay has shown no significant cross-reactivity.

95% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

c-Kit ligand, Kit ligand, KITLG, Mast cell growth factor, MGF, Stem cell factor