Human S100A1 ELISA Kit (A118280)

Human S100A1 ELISA Kit

Human S100A1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human S100A1 in cell culture supernates, serum.

Sandwich (quantitative)

Human S100A1 ELISA Kit (A118280) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human S100A1 in cell culture supernates, serum. An antibody specific for S100A1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the S100A1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-S100A1 Antibody, which binds the captured S100A1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of S100A1 captured in each well. The concentration of S100A1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Cell culture supernates, serum.

0.41 ng/ml

3.13-200 ng/ml

4 hours

100µl

Human

Intra-assay CV: <2.7%, Inter-assay CV: <3.9%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-S100A1 Antibody (A329820) as the capture antibody and Anti-S100A1 Antibody (A329821) as the detection antibody.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

This product is for research use only. It is not intended for diagnostic or therapeutic use.