Human MMP9 ELISA Kit (A117517)

Human MMP9 ELISA Kit

Human MMP9 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MMP9 in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Human MMP9 ELISA Kit (A117517) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human MMP9 in serum, plasma and cell culture supernates. An antibody specific for MMP9 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MMP9 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-MMP9 Antibody, which binds the captured MMP9 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of MMP9 captured in each well. The concentration of MMP9 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

15.2 pg/ml

31.2-2000 pg/ml

4 hours

100µl

Human

Intra-assay CV: <4.2%, Inter-assay CV: <6.9%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-MMP9 Antibody (A329614) as the capture antibody, Anti-MMP9 Antibody (A329615) as the detection antibody, and Recombinant Human MMP9 Protein (C-terminal His Tag) (A331071) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9