Human MMP-9 ELISA Kit (A1493)

Human MMP-9 ELISA Kit

Sandwich (quantitative)

Human MMP-9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-9 in the samples. A monoclonal antibody specific for human MMP-9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MMP-9, and MMP-9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

78.13 - 5000 pg/ml

This kit can assay both natural and recombinant human MMP-9.

Human

This assay has shown no significant cross-reactivity.

97% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9