Human MCP-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MCP-1 in the samples. A monoclonal antibody specific for human MCP-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for MCP-1, and MCP-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MCP-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
2.18 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both natural and recombinant human MCP-1.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
106% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
C-C motif chemokine 2, CCL2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
