Human Leptin ELISA Kit (A1479)

Human Leptin ELISA Kit

Sandwich (quantitative)

Human Leptin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Leptin in the samples. A monoclonal antibody specific for human Leptin has been immobilized onto microwells. Standard or samples are pipetted into the wells, and Leptin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for Leptin is added to the wells. Following a wash to remove any unbound detect antibody, Streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Leptin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

312.50 - 20000 pg/ml

This kit can assay both natural and recombinant human Leptin.

Human

This assay has shown no significant cross-reactivity.

98% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

LEP, OB, Obese protein, Obesity factor, OBS