Human Leptin ELISA Kit (A117480)

Human Leptin ELISA Kit

Human Leptin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Leptin in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Human Leptin ELISA Kit (A117480) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Leptin in serum, plasma and cell culture supernates. An antibody specific for Leptin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Leptin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Leptin Antibody, which binds the captured Leptin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Leptin captured in each well. The concentration of Leptin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

15.6 pg/ml

31.2-2000 pg/ml

4 hours

100µl

Human

Intra-assay CV: <4.1%, Inter-assay CV: <5.8%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-Leptin Antibody (A329563) as the capture antibody, Anti-Leptin Antibody (A329564) as the detection antibody, and Recombinant Human Leptin Protein (A330995) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

LEP, OB, Obese protein, Obesity factor, OBS