Human IL-1ra ELISA Kit (A1459)

Human IL-1ra ELISA Kit

Sandwich (quantitative)

Human IL-1ra ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-1ra in the samples. A monoclonal antibody specific for human IL-1ra has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IL-1ra, and IL-1ra present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-1ra bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

39.06 - 2500 pg/ml

This kit can assay both natural and recombinant human IL-1ra.

Human

This assay has shown no significant cross-reactivity.

101% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

CD210, CDw210a, ICIL-1RA, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R subunit alpha, IL-10R1, IL-10RA, IL-1RN, IL1 inhibitor, IL10R, IL10RA, IL1F3, IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, Interleukin-10 receptor subunit 1, Interleukin-10 receptor subunit alpha, IRAP