Human IL-1 alpha ELISA Kit (A117476)

Human IL-1 alpha ELISA Kit

Human IL-1 alpha ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-1 alpha in serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids.

Sandwich (quantitative)

Human IL-1 alpha ELISA Kit (A117476) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human IL-1 alpha in serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids. An antibody specific for IL-1 alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-1 alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-1 alpha Antibody, which binds the captured IL-1 alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-1 alpha captured in each well. The concentration of IL-1 alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

1.26 pg/ml

7.81-500 pg/ml

4 hours

100µl

Human

Intra-assay CV: <3.5%, Inter-assay CV: <6.3%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-IL-1 alpha Antibody (A329479) as the capture antibody, Anti-IL-1 alpha Antibody (A329480) as the detection antibody, and Recombinant Human IL-1 alpha Protein (C-terminal His Tag) (A330794) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

Hematopoietin-1, IL1A, IL1F1, Interleukin-1 alpha