Human HLA G ELISA Kit (A326887)

Human HLA G ELISA Kit

Human HLA G ELISA Kit is a indirect Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of human HLA G in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Indirect (quantitative)

Human HLA G ELISA Kit (A326887) employs the Indirect ELISA technique for the quantitative measurement of human HLA G in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.. The 96-well microtiter plate has been pre-coated with HLA G antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of HLA G sample captured on the plate. The concentration of HLA G can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

1.875 ng/ml

4 hours

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, HLA-6.0, HLAG, MHC class I antigen G