Human G-CSF ELISA Kit (A573)

$635

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Human G-CSF ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This G-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-G-CSF Antibody. Standards or samples are then added to the microtire plate wells and G-CSF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of G-CSF present in the sample, Anti-G-CSF Antibody (Biotin) is added to each well to "sandwich" the G-CSF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain G-CSF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 4 pg/ml
Product Range
31.2-2000 pg/ml
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
C17orf33, CSF3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Human G-CSF ELISA Kit (A573)? Please let us know so that we can list the citation on this page.

Alternative products to Human G-CSF ELISA Kit (A573)