Human Fas Ligand ELISA Kit (A1427)

Human Fas Ligand ELISA Kit

Sandwich (quantitative)

Human Fas Ligand ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Fas Ligand in the samples. A monoclonal antibody specific for human Fas Ligand has been immobilized onto microwells. Standard or samples are pipetted into the wells, followd by the addition of biotin-linked monoclonal antibody specific for Fas Ligand, and Fas Ligand present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Fas Ligand bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

15.63 - 1000 pg/ml

This kit can assay both natural and recombinant human Fas Ligand.

Human

This assay has shown no significant cross-reactivity.

97% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 ligand, CD95-L, CD95L, Fas antigen ligand, FasL, FASLG, TNFSF6, Tumor necrosis factor ligand superfamily member 6